Nowadays, the oyster mushrooms can be cultivated successfully under semi controlled conditions in a customized space by using agricultural as well as industrial waste and other refuse as substrate. In the Albania, for the first time we conducted grown of Ploreatus Ostreatus mushrooms in the farm in the favorite conditions (like natural conditions) from applied high technology, automatic control for each parameter. This study present content of metals and nutrient in the “Pleurotus Ostretus” mushrooms samples that was cultivated on wheat straw substrate compositions using Atomic Absorption Spectrometry. We are collected a total of 20 samples where 10 samples are edible mushrooms and 10 samples are substrate samples. The mean concentrations of the elements K, Ca, Mn, Fe, Cu, Zn, Rb, Cr, Ni and Pb were determined in mushrooms cultivated on wheat straw substrate and we have calculated bioaccumulation factor substrate to edible mushrooms. All results are presented in table and graphical forms. Also we have determined protein, carbohydrates, fat, acidity and humidity, in the Pleurotus Ostreatus mushrooms.
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